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DIN EN ISO 22959:2009-10

DIN EN ISO 22959:2009-10

Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons by on-line donor-acceptor complex chromatography and HPLC with fluorescence detection (ISO 22959:2009); German version EN ISO 22959:2009



This standard specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils. The method has been validated for coconut (CN), olive (OV), sunflower (SF) and soyabean (BO) oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters. The lowest level of quantification for the PAHs is 0,1 µg/kg. The lowest possible amount of each PAH, which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1 µg/kg to 3,5 µg/kg for each individual PAH. For samples containing (light) PAH contents more than 3,5 µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However ranges exceeding 3,5 µg/kg have not been validated. PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benz[a]anthracene, benz(e)pyrene, benz(a)pyrene, perylene, benz[g,h,i]perylene, anthanthrene, dibenz[a,h]anthracene, coronene, indeno(123-cd)pyrene, benz(a)fluoranthene, Benz(b)fluoranthene, benz(k)fluoranthene.

Additional Info

Author DIN
Published by DIN
Document type Standard
Format File
ICS 67.200.10 : Oilseeds
Number of pages 28
Replace DIN EN ISO 22959 (2007-12)
Cross references BVL L 13.00-43 (2012-01), IDT
Year 2009
Document history DIN EN ISO 22959 (2009-10)
Country Germany
Keyword EN ISO 22959;22959

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